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KMID : 0861020130280040093
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Korea Journal of Herbology
2013 Volume.28 No. 4 p.93 ~ p.102
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Anti-inflammatory and Anti-Pruritonic Effects of WSY-1075 composited with Medicinal Plants on the Activated Rat Peritoneal Mast cells and Mouse Pruritus
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황성연:Hwang Sung-Yeoun
이승호:Lee Seung-Ho/이가위:Lee Chia-Wei/김안나:Kim An-Na/김홍준:Kim Hong-Jun/김장호:Kim Jang-Ho/장선일:Jang Seon-Il
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Abstract
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Objectives : This study was to evaluate the anti-inflammatory and anti-pruritic effects of WSY-1075 composited with Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Ginseng Radix, Cervi parvum Cornu and Cinnamomi Cortex in rat peritoneal mast cells (RPMCs) and scratching mouse model.
Methods : WSY-1075 was prepared by extracting with 30% ethanol. In the present study, we investigated the effect of WSY-1075 on the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and histamine in rat peritoneal mast cells (RPMCs) activated with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187, and on the scratching behavior in mice treated with pruriogens.
Results : WSY-1075 was not cytotoxic effect in used all concentration. PMA plus A23187 treatment significantly increased TNF-α, IL-1β and IL-6 production compared with media control in RPMCs. However, TNF-α, IL1β and IL-6 production increased by PMA plus A23187 treatment were significantly inhibited by WSY-1075 (200 μg/mL and 400 μg/mL). WSY-1075 also inhibited the histamine release from RPMCs stim ulated by compound 48/80, which promotes histamine release. Moreover, WSY-1075 administration had an inhibitory effects on the scratching behavior induced by pruritogen (compound 48/80, histamine, serotonin and substence P) in ICR mice.
Conclusion : These results suggest that WSY-1075 administration (200 mg/kg or 400 mg/kg) has the anti-inflammatory and anti-pruritic effects on the activated rat peritoneal mast cell and mouse pruritus. WSY-1075 has a potential use as a composition of medicinal plants for treatment against inflammation- and pruritus-related disease.
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KEYWORD
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WSY-1075, GongJin-Dan(拱辰丹), Rat mast cells, Anti-inflammation, Anti-pruritus
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